Neoschizomer
Type of restriction enzymes
Neoschizomers are restriction enzymes that recognize the same nucleotide sequence, but cleave at different sites. The first restriction enzyme discovered to recognize a sequence is called the prototype, and others that recognize the same sequence are isoschizomers. Neoschizomers are a subset of isoschizomers.[1]
For example, MaeII is the prototype enzyme for the sequence "ACGT", with the cleavage site A↓CGT. One of its neoschizomers, Tsp49I, also recognizes the sequence "ACGT", but cleaves at ACGT↓.[2]
Another example is SmaI (CCC↓GGG), which is a neoschizomer of XmaI (C↓CCGGG).[3]
Use in molecular biology
External links
- Rebase restriction enzyme database, http://rebase.neb.com/rebase/
See also
Wikidata has the property:
neoschizomer (P4875) (see uses)
- Isoschizomer
- Isocaudomer
- List of restriction enzyme cutting sites
References
- ^ "Isoschizomers". New England Biolabs. Retrieved 2024-09-12.
- ^ Welch, S. (1996-05-15). "Tsp49I (ACGT↓), a Thermostable Neoschizomer of the Type II Restriction Endonuclease Mae II (A↓CGT), Discovered in Isolates of the Genus Thermus from the Azores, Iceland and New Zealand". Nucleic Acids Research. 24 (10): 1799–1801. doi:10.1093/nar/24.10.1799. PMC 145888. PMID 8657557.
- ^ Withers, Barbara E.; Dunbar, Joan C. (1995). "DNA determinants in sequence-specific recognition by Xm al endonuclease". Nucleic Acids Research. 23 (17): 3571–3577. doi:10.1093/nar/23.17.3571. PMC 307239. PMID 7567471.
- v
- t
- e
Restriction modification system: restriction enzyme
- Isoschizomer
- Neoschizomer
- Isocaudomer
- TaqI
- Sau3A
- AluI*
- HaeIII*
- DpnII
- NlaIII
- HpaII
- EcoRII
- FokI
- AaaI
- BglII
- PovII*
- EcoRV*
- EcoRI
- BamHI
- HindIII
- XbaI
- XhoI
- SacI
- PstI
- NdeI
- NotI
- A
- Ba–Bc
- Bd–Bp
- Bsa–Bso
- Bsp–Bss
- Bst–Bv
- C-D
- E-F
- G-K
- L-N
- O-R
- S
- T-Z
* means cleavage produces blunt ends
 | This enzyme-related article is a stub. You can help Wikipedia by expanding it. |
- v
- t
- e
