MKI67IP

Protein-coding gene in humans
NIFK
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

2AFF

Identifiers
AliasesNIFK, MKI67IP, Nopp34, nucleolar protein interacting with the FHA domain of MKI67
External IDsOMIM: 611970; MGI: 1915199; HomoloGene: 49862; GeneCards: NIFK; OMA:NIFK - orthologs
Gene location (Human)
Chromosome 2 (human)
Chr.Chromosome 2 (human)[1]
Chromosome 2 (human)
Genomic location for NIFK
Genomic location for NIFK
Band2q14.3Start121,726,945 bp[1]
End121,736,911 bp[1]
Gene location (Mouse)
Chromosome 1 (mouse)
Chr.Chromosome 1 (mouse)[2]
Chromosome 1 (mouse)
Genomic location for NIFK
Genomic location for NIFK
Band1|1 E2.3Start118,249,569 bp[2]
End118,261,552 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • Achilles tendon

  • ventricular zone

  • ganglionic eminence

  • cartilage tissue

  • tibialis anterior muscle

  • mucosa of sigmoid colon

  • body of pancreas

  • lymph node

  • endometrium

  • appendix
Top expressed in
  • primitive streak

  • genital tubercle

  • epiblast

  • embryo

  • embryo

  • tail of embryo

  • morula

  • morula

  • yolk sac

  • ventricular zone
More reference expression data
BioGPS
More reference expression data
Gene ontology
Molecular function
  • protein binding
  • RNA binding
  • nucleic acid binding
Cellular component
  • cytoplasm
  • condensed nuclear chromosome
  • chromosome
  • nucleolus
  • nucleus
  • nucleoplasm
Biological process
  • negative regulation of phosphatase activity
  • rRNA transcription
  • rRNA metabolic process
  • protein-containing complex assembly
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

84365

67949

Ensembl

ENSG00000155438

ENSMUSG00000026377

UniProt

Q9BYG3

Q91VE6

RefSeq (mRNA)

NM_032390

NM_026472

RefSeq (protein)

NP_115766

NP_080748

Location (UCSC)Chr 2: 121.73 – 121.74 MbChr 1: 118.25 – 118.26 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse
FHA Ki67 binding domain of hNIFK
the solution structure of the ki67fha/hnifk(226-269)3p complex
Identifiers
SymbolhNIFK_binding
PfamPF12196
InterProIPR021043
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary

MKI67 FHA domain-interacting nucleolar phosphoprotein is a protein that in humans is encoded by the MKI67IP gene.[5][6]

MKI67 FHA domain-interacting nucleolar phosphoprotein contains an RNA recognition motif (RRM) near to the N-terminus and a FHA Ki67 binding domain near to the C-terminus. There are two conserved sequence motifs within the FHA Ki67 binding domain: TPVCTP and LERRKS, this domain binds to the forkhead-associated domain of human Ki67. High-affinity binding requires sequential phosphorylation by two kinases, CDK1 and GSK3, yielding pThr238, pThr234 and pSer230. This interaction is involved in cell cycle regulation.[7]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000155438 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026377 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Takagi M, Sueishi M, Saiwaki T, Kametaka A, Yoneda Y (Jul 2001). "A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis". J Biol Chem. 276 (27): 25386–91. doi:10.1074/jbc.M102227200. PMID 11342549.
  6. ^ "Entrez Gene: MKI67IP MKI67 (FHA domain) interacting nucleolar phosphoprotein".
  7. ^ Byeon IJ, Li H, Song H, Gronenborn AM, Tsai MD (November 2005). "Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67". Nat. Struct. Mol. Biol. 12 (11): 987–93. doi:10.1038/nsmb1008. PMID 16244663. S2CID 5680428.

Further reading

  • Scherl A, Couté Y, Déon C, et al. (2003). "Functional proteomic analysis of human nucleolus". Mol. Biol. Cell. 13 (11): 4100–9. doi:10.1091/mbc.E02-05-0271. PMC 133617. PMID 12429849.
  • Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
  • Li H, Byeon IJ, Ju Y, Tsai MD (2004). "Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions". J. Mol. Biol. 335 (1): 371–81. doi:10.1016/j.jmb.2003.10.032. PMID 14659764.
  • Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
  • Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
  • Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724–31. Bibcode:2005Natur.434..724H. doi:10.1038/nature03466. PMID 15815621.
  • Byeon IJ, Li H, Song H, et al. (2006). "Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67". Nat. Struct. Mol. Biol. 12 (11): 987–93. doi:10.1038/nsmb1008. PMID 16244663. S2CID 5680428.
  • Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. Bibcode:2006PNAS..103.5391N. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
  • PDBe-KB provides an overview of all the structure information available in the PDB for Human MKI67 FHA domain-interacting nucleolar phosphoprotein (MKI67IP)


  • v
  • t
  • e
  • 2aff: The solution structure of the Ki67FHA/hNIFK(226-269)3P complex
    2aff: The solution structure of the Ki67FHA/hNIFK(226-269)3P complex
This article incorporates text from the public domain Pfam and InterPro: IPR021043


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