LRRC23

LRRC23
Identifiers
AliasesLRRC23, LRPB7, leucine rich repeat containing 23
External IDsMGI: 1315192; HomoloGene: 5082; GeneCards: LRRC23; OMA:LRRC23 - orthologs
Gene location (Human)
Chromosome 12 (human)
Chr.Chromosome 12 (human)[1]
Chromosome 12 (human)
Genomic location for LRRC23
Genomic location for LRRC23
Band12p13.31Start6,873,569 bp[1]
End6,914,241 bp[1]
Gene location (Mouse)
Chromosome 6 (mouse)
Chr.Chromosome 6 (mouse)[2]
Chromosome 6 (mouse)
Genomic location for LRRC23
Genomic location for LRRC23
Band6 F2|6 59.17 cMStart124,746,826 bp[2]
End124,756,690 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • right uterine tube

  • bronchial epithelial cell

  • olfactory zone of nasal mucosa

  • left testis

  • nasal epithelium

  • right testis

  • mucosa of paranasal sinus

  • caudate nucleus

  • sural nerve

  • nucleus accumbens
Top expressed in
  • spermatocyte

  • spermatid

  • seminiferous tubule

  • left lung lobe

  • right lung lobe

  • olfactory epithelium

  • choroid plexus of fourth ventricle

  • morula

  • Epithelium of choroid plexus

  • neural layer of retina
More reference expression data
BioGPS
More reference expression data
Gene ontology
Molecular function
  • molecular function
Cellular component
  • cellular component
  • cytoplasm
  • cytosol
Biological process
  • biological process
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

10233

16977

Ensembl

ENSG00000010626

ENSMUSG00000030125

UniProt

Q53EV4

O35125

RefSeq (mRNA)

NM_201650
NM_001135217
NM_006992
NM_181613

NM_013588
NM_001302555

RefSeq (protein)

NP_001128689
NP_008923
NP_964013

NP_001289484

Location (UCSC)Chr 12: 6.87 – 6.91 MbChr 6: 124.75 – 124.76 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Leucine-rich repeat-containing protein 23 is a protein that in humans is encoded by the LRRC23 gene.[5][6][7]

Function

The function of LRRC23 is unknown. It is a member of the leucine-rich repeat family of proteins, which are known for participating in protein-protein interactions. Experimental evidence suggests that LRRC23 interacts with the CD28 protein in a pathway related to the immune system and development of regulatory T cells that control spontaneous autoimmune disease.[8]

Protein sequence

LRRC23 spans 343 residues containing two varieties of internally repeating sequence. Detected and aligned by RADAR,[9] the most abundant repeat is the leucine-rich repeat, repeating 9 times in bases 89-287. The other repeated sequence occurs twice in bases 3-36. The RADAR program output, below, summarizes the composition and location of all the repeats and aligns them for comparison against each other.

The human genome produces three known variants of LRRC23.[7] The largest splice variant, variant 3, contains 8 exons. Variants 1 and 2 use alternative first exons, and variant 2 excludes the seventh exon, giving it a total of seven exons making up the mRNA.

Protein structure

Although the actual structure of LRRC23 is unknown, comparison to the crystal structures of various similar proteins such as 2OMW A (e-value 1.00e-17) reveals a structure typical of other leucine-rich repeat proteins. Alternating beta sheets and coils create a spiraled peptide chain forming an arch shape with beta-sheets occupying the concave surface.[10]

The aligned structure of 2OMW_A with LRRC23 spans acids 72-272 of the LRRC23 protein. Conserved asparagines are highlighted in yellow, showing the regularity of spacing and repeat structure within. This model was generated using Cn3D software provided by NCBI.

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000010626 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000030125 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Tzachanis D, Berezovskaya A, Nadler LM, Boussiotis VA (Feb 2002). "Blockade of B7/CD28 in mixed lymphocyte reaction cultures results in the generation of alternatively activated macrophages, which suppress T-cell responses". Blood. 99 (4): 1465–73. doi:10.1182/blood.V99.4.1465. PMID 11830501.
  6. ^ Chang TT, Kuchroo VK, Sharpe AH (Feb 2002). "Role of the B7-CD28/CTLA-4 pathway in autoimmune disease". Signal Transduction Pathways in Autoimmunity. Current Directions in Autoimmunity. Vol. 5. pp. 113–30. doi:10.1159/000060550. ISBN 3-8055-7308-1. PMID 11826754.
  7. ^ a b "Entrez Gene: LRRC23 leucine rich repeat containing 23".
  8. ^ Salomon B, Lenschow DJ, Rhee L, Ashourian N, Singh B, Sharpe A, Bluestone JA (Apr 2000). "B7/CD28 costimulation is essential for the homeostasis of the CD4+CD25+ immunoregulatory T cells that control autoimmune diabetes". Immunity. 12 (4): 431–40. doi:10.1016/S1074-7613(00)80195-8. PMID 10795741.
  9. ^ RADAR: European Molecular Biology Laboratory - European Bioinformatics Institute (EMBLE-EBI) Radar program: [1]
  10. ^ NCBI Structure CBlast https://www.ncbi.nlm.nih.gov/Structure/cblast/cblast.cgi?client=entrez&query_gi=206725447&hit=149242643&hsp=1&output=html&pagenum=1&epp=20&sort=evalue&view=graphic&subset=allmmdb

Further reading

  • Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
  • Ansari-Lari MA, Muzny DM, Lu J, Lu F, Lilley CE, Spanos S, Malley T, Gibbs RA (Apr 1996). "A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13". Genome Research. 6 (4): 314–26. doi:10.1101/gr.6.4.314. PMID 8723724.
  • Bonaldo MF, Lennon G, Soares MB (Sep 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
  • Ansari-Lari MA, Shen Y, Muzny DM, Lee W, Gibbs RA (Mar 1997). "Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination". Genome Research. 7 (3): 268–80. doi:10.1101/gr.7.3.268. PMID 9074930.
  • Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
  • Tasheva ES, An K, Boyle DL, Conrad GW (2006). "Expression and localization of leucine-rich B7 protein in human ocular tissues". Molecular Vision. 11: 452–60. PMID 16030496.


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