Protein-coding gene in the species Homo sapiens
| KIFAP3 |
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| Identifiers |
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| Aliases | KIFAP3, FLA3, KAP-1, KAP-3, KAP3, SMAP, Smg-GDS, dJ190I16.1, kinesin associated protein 3 |
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| External IDs | OMIM: 601836; MGI: 107566; HomoloGene: 7799; GeneCards: KIFAP3; OMA:KIFAP3 - orthologs |
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| Gene location (Human) |
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 | | Chr. | Chromosome 1 (human)[1] |
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| | Band | 1q24.2 | Start | 169,921,326 bp[1] |
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| End | 170,085,208 bp[1] |
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| Gene location (Mouse) |
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 | | Chr. | Chromosome 1 (mouse)[2] |
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| | Band | 1|1 H2.1 | Start | 163,607,152 bp[2] |
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| End | 163,744,678 bp[2] |
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| RNA expression pattern |
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| Bgee | | Human | Mouse (ortholog) |
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| Top expressed in | - pons
- orbitofrontal cortex
- lateral nuclear group of thalamus
- Brodmann area 46
- frontal pole
- postcentral gyrus
- prefrontal cortex
- ganglionic eminence
- sperm
- dorsolateral prefrontal cortex
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| | Top expressed in | - barrel cortex
- facial motor nucleus
- nucleus accumbens
- prefrontal cortex
- subiculum
- pontine nuclei
- temporal lobe
- medial dorsal nucleus
- anterior horn of spinal cord
- superior colliculus
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| | More reference expression data |
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| BioGPS |  | | More reference expression data |
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| Gene ontology |
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| Molecular function | - protein binding
- kinesin binding
- intraciliary transport particle B binding
- protein phosphatase binding
| | Cellular component | - intraciliary transport particle
- ciliary basal body
- cytosol
- centrosome
- Golgi apparatus
- kinesin complex
- microtubule cytoskeleton
- kinesin II complex
- photoreceptor connecting cilium
- condensed nuclear chromosome
- ciliary tip
- spindle microtubule
- endoplasmic reticulum
- periciliary membrane compartment
- axoneme
- cilium
| | Biological process | - protein localization
- plus-end-directed vesicle transport along microtubule
- antigen processing and presentation of exogenous peptide antigen via MHC class II
- negative regulation of apoptotic process
- microtubule-based movement
- positive regulation of calcium-dependent cell-cell adhesion
- microtubule-based process
- signal transduction
- negative regulation of cell population proliferation
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
- intraciliary transport involved in cilium assembly
- protein-containing complex assembly
| | Sources:Amigo / QuickGO |
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| Orthologs |
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| Species | Human | Mouse |
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| Entrez | | |
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| Ensembl | | |
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| UniProt | | |
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| RefSeq (mRNA) | NM_001204514
NM_001204516
NM_001204517
NM_014970
NM_001375830
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NM_001375831 |
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| RefSeq (protein) | NP_001191443
NP_001191445
NP_001191446
NP_055785
NP_001362759
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NP_001362760 |
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| Location (UCSC) | Chr 1: 169.92 – 170.09 Mb | Chr 1: 163.61 – 163.74 Mb |
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| PubMed search | [3] | [4] |
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| Wikidata |
| View/Edit Human | View/Edit Mouse |
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Kinesin-associated protein 3 (KAP3) is a protein that in humans is encoded by the KIFAP3 gene.[5][6] It is a non-motor, accessory subunit which co-oligomerizes with the motor subunits KIF3A and KIF3B or KIF3C, to form heterotrimeric kinesin-2 motor proteins. Kinesin-2 KAP subunits were initially characterized in echinoderms and mice.[7][8]
Function
The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 Armadillo repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein.[6] It has also been proposed to act as a clamp, stabilizing the C-terminal half [9] of the otherwise unstable stalk coiled-coil.
Interactions
KIFAP3 has been shown to interact with APC,[10] SMC3[11] and RAP1GDS1.[5]
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000075945 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026585 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ a b Shimizu K, Kawabe H, Minami S, Honda T, Takaishi K, Shirataki H, Takai Y (Dec 1996). "SMAP, an Smg GDS-associating protein having arm repeats and phosphorylated by Src tyrosine kinase". J Biol Chem. 271 (43): 27013–7. doi:10.1074/jbc.271.43.27013. PMID 8900189.
- ^ a b "Entrez Gene: KIFAP3 kinesin-associated protein 3".
- ^ Wedaman KP, Meyer DW, Rashid DJ, Cole DG, Scholey JM (February 1996). "Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex". J. Cell Biol. 132 (3): 371–80. doi:10.1083/jcb.132.3.371. PMC 2120715. PMID 8636215.
- ^ Yamazaki H, Nakata T, Okada Y, Hirokawa N (August 1996). "Cloning and characterization of KAP3: a novel kinesin superfamily-associated protein of KIF3A/3B". Proc. Natl. Acad. Sci. U.S.A. 93 (16): 8443–8. Bibcode:1996PNAS...93.8443Y. doi:10.1073/pnas.93.16.8443. PMC 38690. PMID 8710890.
- ^ Ahmed Z, Mazumdar S, Ray K (February 2020). "Kinesin associated protein, DmKAP, binding harnesses the C-terminal ends of the Drosophila kinesin-2 stalk heterodimer". Biochem Biophys Res Commun. 522 (2): 506–511. doi:10.1016/j.bbrc.2019.11.111. PMC 8407544. PMID 31784087.
- ^ Jimbo T, Kawasaki Y, Koyama R, Sato R, Takada S, Haraguchi K, Akiyama T (April 2002). "Identification of a link between the tumour suppressor APC and the kinesin superfamily". Nat. Cell Biol. 4 (4): 323–7. doi:10.1038/ncb779. PMID 11912492. S2CID 10745049.
- ^ Shimizu K, Shirataki H, Honda T, Minami S, Takai Y (March 1998). "Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide". J. Biol. Chem. 273 (12): 6591–4. doi:10.1074/jbc.273.12.6591. PMID 9506951.
Further reading
- Nagata K, Puls A, Futter C, et al. (1998). "The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3". EMBO J. 17 (1): 149–58. doi:10.1093/emboj/17.1.149. PMC 1170366. PMID 9427749.
- Shimizu K, Shirataki H, Honda T, et al. (1998). "Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide". J. Biol. Chem. 273 (12): 6591–4. doi:10.1074/jbc.273.12.6591. PMID 9506951.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Khanna H, Hurd TW, Lillo C, et al. (2005). "RPGR-ORF15, which is mutated in retinitis pigmentosa, associates with SMC1, SMC3, and microtubule transport proteins". J. Biol. Chem. 280 (39): 33580–7. doi:10.1074/jbc.M505827200. PMC 1249479. PMID 16043481.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
- Brown CL, Maier KC, Stauber T, et al. (2006). "Kinesin-2 is a motor for late endosomes and lysosomes". Traffic. 6 (12): 1114–24. doi:10.1111/j.1600-0854.2005.00347.x. PMID 16262723.
- Haraguchi K, Hayashi T, Jimbo T, et al. (2006). "Role of the kinesin-2 family protein, KIF3, during mitosis". J. Biol. Chem. 281 (7): 4094–9. doi:10.1074/jbc.M507028200. PMID 16298999.
- Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315–21. Bibcode:2006Natur.441..315G. doi:10.1038/nature04727. PMID 16710414.
- Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569. S2CID 13709685.
- Kuraguchi M, Wang XP, Bronson RT, et al. (2006). "Adenomatous Polyposis Coli (APC) Is Required for Normal Development of Skin and Thymus". PLOS Genet. 2 (9): e146. doi:10.1371/journal.pgen.0020146. PMC 1564426. PMID 17002498.
- Stauber T, Simpson JC, Pepperkok R, Vernos I (2007). "A role for kinesin-2 in COPI-dependent recycling between the ER and the Golgi complex". Curr. Biol. 16 (22): 2245–51. doi:10.1016/j.cub.2006.09.060. PMID 17113389. S2CID 9739953.
